Diagnostic Value of Serum CXCL2 and Fibronectin-1 in Acute Myocardial Infarction and Their Association with Neutrophil Extracellular Trap Formation.

ObjectiveBased on bioinformatics screening, C-X-C motif chemokine ligand 2 (CXCL2) and fibronectin 1 (FN1) were identified as common targets of acute myocardial infarction (AMI) and neutrophil extracellular traps (NETs). This study aimed to validate their adjunctive differential diagnostic value in emergency department patients with chest pain suspected of AMI, and explore NET associations.MethodsIn this prospective observational study, 349 patients with chest pain suspected of AMI were enrolled between June 2022 and January 2025 and classified as non-AMI (n=226) or AMI (n=123). Serum CXCL2, FN1, and NETs biomarkers (myeloperoxidase-DNA [MPO-DNA] and citrullinated histone H3 [CitH3]) were measured by enzyme-linked immunosorbent assay (ELISA). Multivariable logistic regression, receiver operating characteristic (ROC) curves, and Spearman correlation were performed.ResultsAMI patients had higher proportions of males, smokers, and angina, with elevated heart rate, fasting blood glucose (FBG), glycated hemoglobin (HbA1c), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR), and reduced high-density lipoprotein cholesterol (HDL-C) versus non-AMI (P