From qPCR to Next-Generation Sequencing: Practical Strategies for Resource-Limited Molecular Laboratories.

Personalized medicine, also referred to as precision medicine, has been enabled by emerging technologies such as next-generation sequencing (NGS), particularly through applications like liquid biopsy, where genetics and genomics play a central role. Consequently, the integration of NGS into clinical and research settings has become increasingly essential. However, incorporating NGS into molecular biology platforms traditionally based on quantitative polymerase chain reaction (qPCR) represents a significant challenge in Algeria and other resource-limited settings. This transition requires substantial adaptations in laboratory infrastructure and workflow design, as well as the implementation of robust quality management systems. Additional challenges include personnel training, assay validation, data management, regulatory compliance, cost sustainability, and workforce development. This literature review aims to identify and analyze potential solutions to the challenges associated with implementing NGS within qPCRbased molecular biology platforms in resource-limited countries, with a particular focus on Algeria.