Abstract / Description
ABSTRACT Frequent blood testing remains invasive and impractical, hindering real‐time health monitoring. Interstitial fluid (ISF) offers a promising alternative, yet current microneedle platforms are complex, inefficient, and lack multiplexing capability. Here, we present a portable and dual‐button microneedle device for rapid ISF sampling, coupled with multimodal laser sensing for molecular and elemental analysis. The disposable device has a low material cost (< 2 USD). The dual‐button design ensures user‐friendly microneedle operation, while the hollow microneedles with built‐in microfluidic channels enable ISF sampling within 1 min. In parallel, gold nanocubes coupled with MXene are engineered as a sensing module, providing signal enhancement for laser spectroscopy in molecular and elemental analysis. Furthermore, artificial intelligence (AI)‐assisted data processing enhances spectral data interpretation for comprehensive health assessment. Both in vitro and in vivo studies demonstrate promising performance (with accuracies exceeding 88%). This user‐friendly, rapid, cost‐effective, and multiplexed device offers a powerful route toward clinical translation, bridging the gap between advanced laser spectroscopy and point‐of‐care applications.